Immunofluorescent analysis of Hela cells using CSB-PA017644LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Western Blot Positive WB detected in: HepG2 whole cell lysate, Rat kidney tissue All lanes: PCSK5 antibody at 6µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 207, 102 kDa Observed band size: 102 kDa
IHC image of CSB-PA017644LA01HU diluted at 1:300 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Supplier Page from CUSABIO Technology LLC for PCSK5 Antibody